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Abstract. The symbiosis between host anemones and anemonefish species, although often simplified and romanticized by popular media, demands additional field observation in order to expand scientific understanding of these complex relationships. The association between anemones and anemonefish has been frequently noted in the literature, but no studies have yet been conducted to examine the species-specificity of this symbiosis. Our study examined 3 common anemonefish species and 4 common anemone species native to Malohom Bay, Pulau Gaya, Borneo, Malaysia. Randomized 5m wide transects perpendicular to the shoreline were surveyed, and 27 anemone and anemonefish species pairings were recorded. Pearson's Chi-squared tests for count data were performed on fish species vs. anemone species and fish species vs. relative anemone tentacle length (classed in 3 sizes: short, medium, and long). The p-values for both tests were significant at the 99% confidence level (p < 0.01 for both comparisons), indicating that there is highly significant species-specificity between anemone and anemonefish species and between anemone tentacle length and anemonefish species. Observation of this close association has important ramifications regarding the vulnerability of these symbiotic partnerships to reef degradation; further research could be conducted to determine whether these species-specific relationships result from adaptations of the physiology or the morphology of the fish and/or anemone.
Keyword: anemone, anemonefish, symbiosis, species specificity, reef ecology
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